Impact of water stress on sucrose metabolism in rice (oryza sativa L.)
By: Anie Thomas.
Contributor(s): Beena, R (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Biotechnology, College of Agriculture 2021Description: 66p.Subject(s): Plant Biotechnology | Rice | Oryza sativa L | Sucrose metabolism | Relative expressionDDC classification: 660.6 Online resources: Click here to access online Dissertation note: M Sc Summary: The study entitled “Impact of water stress on sucrose metabolism in rice (Oryza
sativa L.)” conducted at Department of Plant Biotechnology and Department of
Plant Physiology, College of Agriculture, Vellayani during 2020-21. The objective
was to study the impact of water stress on sucrose metabolism by analyzing the
physiological and biochemical parameters and gene expression in selective drought
tolerant and susceptible rice genotypes.
In this study, two rice varieties, drought tolerant variety, PTB 7 (Parambuvattan)
and drought susceptible, PTB 23 (Cheriya Aryan) were grown in pot culture and
after the panicle initiation stage, crops were subjected to water stress by
withdrawing irrigation until the plants experienced the symptoms of stress (leaf
rolling). Then the various physiological parameters were studied five days after the
induction of water stress. Extraction and estimation of sucrose metabolizing
enzymes such as invertase, α- and β-amylase were done five days after induction of
stress spectrophotometrically. Expression levels of sucrose synthase (SuSy) and
sucrose transporter gene (SUC2) were analyzed from both root and leaf seven days
after induction of water stress.
Under water stress, physiological parameters such as cell membrane stability index,
relative water content and yield trait like number of productive tillers were
significantly reduced, activity of enzymes such as invertase, α- and β-amylase were
increased and gene expression level of sucrose synthase (SuSy) and sucrose
transporter (SUC-2), which are associated with the sucrose metabolism were
upregulated. Water stress enhanced the sucrose content and reducing sugar content
in rice plant. There was significant increase in root traits in PTB 7 but they were
reduced in PTB 23. Also, there was a reduction in shoot biomass than the root
biomass in tolerant rice variety, which leads to an increase in root to shoot (R/S)
ratio.
In this study, sucrose metabolism and transport were increased in both drought
tolerant and susceptible variety under water stress condition. However, PTB 7
(drought tolerant) showed an improved sucrose metabolism than PTB 23 (drought
susceptible) during water stress condition.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | Reference Book | 660.6 ANI/IM PG (Browse shelf) | Available | 175222 |
M Sc
The study entitled “Impact of water stress on sucrose metabolism in rice (Oryza
sativa L.)” conducted at Department of Plant Biotechnology and Department of
Plant Physiology, College of Agriculture, Vellayani during 2020-21. The objective
was to study the impact of water stress on sucrose metabolism by analyzing the
physiological and biochemical parameters and gene expression in selective drought
tolerant and susceptible rice genotypes.
In this study, two rice varieties, drought tolerant variety, PTB 7 (Parambuvattan)
and drought susceptible, PTB 23 (Cheriya Aryan) were grown in pot culture and
after the panicle initiation stage, crops were subjected to water stress by
withdrawing irrigation until the plants experienced the symptoms of stress (leaf
rolling). Then the various physiological parameters were studied five days after the
induction of water stress. Extraction and estimation of sucrose metabolizing
enzymes such as invertase, α- and β-amylase were done five days after induction of
stress spectrophotometrically. Expression levels of sucrose synthase (SuSy) and
sucrose transporter gene (SUC2) were analyzed from both root and leaf seven days
after induction of water stress.
Under water stress, physiological parameters such as cell membrane stability index,
relative water content and yield trait like number of productive tillers were
significantly reduced, activity of enzymes such as invertase, α- and β-amylase were
increased and gene expression level of sucrose synthase (SuSy) and sucrose
transporter (SUC-2), which are associated with the sucrose metabolism were
upregulated. Water stress enhanced the sucrose content and reducing sugar content
in rice plant. There was significant increase in root traits in PTB 7 but they were
reduced in PTB 23. Also, there was a reduction in shoot biomass than the root
biomass in tolerant rice variety, which leads to an increase in root to shoot (R/S)
ratio.
In this study, sucrose metabolism and transport were increased in both drought
tolerant and susceptible variety under water stress condition. However, PTB 7
(drought tolerant) showed an improved sucrose metabolism than PTB 23 (drought
susceptible) during water stress condition.
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